Cadmium Uptake from Apical Membrane of LLC-PK Cells via Inorganic Anion Exchanger
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چکیده
منابع مشابه
Molecular modulation of inward and outward apical transporters of L-dopa in LLC-PK(1) cells.
The present study examined the nature of the apical inward and outward L-3,4-dihydroxyphenylalanine (L-dopa) transporters in LLC-PK(1) cells and whether protein kinases differentially modulate the activities of these transporters. The apical inward transfer of L-dopa was promoted through an energy-dependent and sodium-insensitive transporter (Michaelis constant = 38 microM; maximum velocity = 2...
متن کاملApical and basolateral uptake and intracellular fate of dopamine precursor l-dopa in LLC-PK1 cells.
The present study was aimed at the uptake ofl-3,4-dihydroxyphenylalanine (l-dopa) and its intracellular decarboxylation to dopamine. The accumulation ofl-dopa from the apical side in cells cultured in collagen-treated plastic was found to be a saturable process with a Michaelis constant ( K m) of 123 ± 17 μM and a maximal velocity ( V max) of 6.0 ± 0.2 nmol ⋅ mg protein-1 ⋅ 6 min-1. The uptake ...
متن کاملAlbumin overload induces apoptosis in LLC-PK(1) cells.
The degree of albuminuria is a well-known adverse prognostic indicator in human glomerular diseases. However, the mechanisms by which albuminuria by itself contributes to tubulointerstitial injury and progression of renal disease remain unclear. We tested the hypothesis that apoptosis may represent one of the mechanisms by which tubule epithelial cells are damaged after albumin overload in vitr...
متن کاملThe protection of selenium on ROS mediated-apoptosis by mitochondria dysfunction in cadmium-induced LLC-PK(1) cells.
Selenium, an essential trace element, showed the significant protective effects against liver and kidney damage induced by some heavy metals. However, the mechanism how selenium suppresses cadmium (Cd)-induced cytotoxicity remains unclear. In this study, we investigated the protective mechanism of selenium on Cd-induced apoptosis in LLC-PK(1) cells via reactive oxygen species (ROS) and mitochon...
متن کاملAldosterone-stimulated sodium uptake by apical membrane vesicles from A6 cells.
Sodium fluxes in plasma membrane vesicles prepared from the cultured toad kidney epithelial cell line A6 are studied. The vesicles are enriched 7-10 times in apical membrane markers. Sodium uptake is osmotically sensitive and inhibited by low concentrations of amiloride (K0.5 = 7 X 10(-8) M at 1 mM NaCl). Vesicles prepared from aldosterone-treated cells (4.5 h at 10(-7) M aldosterone) show a 2-...
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ژورنال
عنوان ژورنال: Pharmacology & Toxicology
سال: 1998
ISSN: 0901-9928,1600-0773
DOI: 10.1111/j.1600-0773.1998.tb01430.x